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In vivo microinjection
In vivo microinjection is a technique used to introduce foreign molecules, such as DNA, RNA, or proteins, directly into cells or tissues within a living organism using a fine glass needle or micropipette. This method is widely used in molecular biology, developmental biology, and gene therapy, as it allows for precise delivery of genetic material…
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In vivo nanoparticle-based transfection
In vivo nanoparticle-based transfection is a method used to introduce genetic material, such as DNA or RNA, into cells within a living organism using nanoparticles as carriers. This approach is employed in gene therapy and other biomedical applications, as it offers a non-viral alternative for delivering therapeutic nucleic acids into target cells. Nanoparticles are tiny…
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In vivo CRISPR/Cas9 gene editing
In vivo CRISPR/Cas9 gene editing is a powerful technique used to modify the genetic material of living organisms directly within their own cells. CRISPR/Cas9, which stands for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and associated protein 9 (Cas9), is a versatile genome editing tool derived from a bacterial immune system. It has revolutionized the…
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Genes introduction
A gene is a segment of DNA that codes for a specific protein. Proteins are the building blocks of the human body and perform many vital functions. Genes are passed down from parents to offspring and are responsible for the traits inherited. Mutations, or changes in the DNA, can occur in genes and can be…
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DNA introduction
DNA is a molecule that contains the genetic instructions used in the development and function of all known living organisms and many viruses. These instructions are stored as a code made up of four chemical bases: adenine (A), thymine (T), cytosine (C), and guanine (G). The code specifies the sequence of the building blocks, called…
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In vivo transfection overview
How does in vivo transfection work? In vivo transfection allows for the delivery of DNA, RNAi, or mRNA molecules into living organisms and can be performed using two methods, which are: viral or nonviral methods. This application is considered to be more complex than in vitro transfection. However, in vivo transfection is rapidly emerging as a very essential tool in…