Physical Transfection Method

These are the following physical transfection methods which are non-viral and show different transfection efficacy. A few methods are elaborated below for understanding.   

Electroporation

Cell membrane permeability is the diffusion of the molecules through the cell membrane. The electroporation physical method uses the electric current (voltages), which increases the permeability of the cell membrane, and the genetic material easily enters the host cell. This physical method is implemented on the B-cells, primary cells, and stem cells. It sometimes causes cell death.   

Microinjection 

This method is used for single cells such as embryonic stem cells. The DNA and RNA are directly injected into the host cell cytoplasm or nucleus with the help of the microinjector and micropipette.  Sonoporation 

The sonoporation transfection method creates holes in the cell membrane, and the genetic material easily passes through these holes. These holes are created with the help of the microbubble. The microbubble is used to form pores in the cell membrane. That is why it is used in sonoporation. It is also known as cellular sonication.   

Laser Irradiation 

In the laser irradiation transfection method, the laser beam creates small holes in the plasma membrane. These holes allow the entry of nucleic acid into the host cell. It is one of the promising methods in the transfection technique.   

Biolistic Particle Delivery 

Microparticles are involved in this method. The microparticles carry the DNA or RNA and enter the host cell. Nanoparticles are also involved in this procedure. 

Commercial In Vivo Transfection Services

Offered as preclinical research services by Altogen Labs. Company is a GLP-compliant contract research lab that provides transfection services (RNAi, CRISPR, etc) and validated xenograft models (CDX, PDX, Syngeneic, etc). Their gene expression analysis supported by RT-PCR (mRNA) and quantitative Western Blot (protein expression).